Article
The evaluation of factor VIII binding activity of von Willebrand factor by means of an ELISA method: significance and practical implications.
American journal of clinical pathology - 1 Mar 1998
Casonato A, Pontara E, Zerbinati P, Zucchetto A, Girolami A
Abstract excerpt
One of the functions of von Willebrand factor (vWF) is to serve as a carrier of clotting factor VIII (FVIII). Deficiency of this function results in the von Willebrand disease (vWD) variant type 2N, which resembles hemophilia A. We describe a new sandwich enzyme-linked immunosorbent assay (ELISA) to study the ability of vWF to bind exogenous recombinant FVIII (rFVIII), in which anti-vWF-coated plates are...
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