Article
Exhaustive scanning approach to screen all the mitochondrial tRNA genes for mutations and its application to the investigation of 35 independent patients with mitochondrial disorders.
Human molecular genetics - 1 Jan 1998
Sternberg D, Danan C, Lombès A, Laforêt P, Girodon E, Goossens M, Amselem S
Abstract excerpt
To gain a better understanding of the molecular basisof mitochondrial (mt) encephalomyopathies, a highly heterogeneous condition, we developed a denaturing gradient gel electrophoresis-based approach that allows rapid and exhaustive screening for mutations of all 22 mt tRNA-encoding genes and the...
Topics
- DNA, Mitochondrial
- Humans
- Mitochondrial Encephalomyopathies
- Mutation
- Polymerase Chain Reaction
- Polymorphism, Genetic
- RNA, Transfer