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Use of multiplex PCR for simultaneous detection of four bacterial species in middle ear effusions

1 Nov 1997

Abstract excerpt

A multiplex PCR procedure was developed for the simultaneous detection of Alloiococcus otitidis, Haemophilus influenzae, Moraxella catarrhalis, and Streptococcus pneumoniae in middle ear effusions (MEEs) from patients with chronic otitis media with effusion. The bacterial 16S rRNA gene was chosen as the target, and the procedure used one common lower primer and four species-specific upper primers. The reaction...

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PMID: 9350746
DOI: 10.1128/jcm.35.11.2854-2858.1997

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