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Article

Multiplex polymerase chain reaction assay for genotyping Clostridium perfringens.

American journal of veterinary research - 1 Jul 1997

Meer R R, Songer J G

Abstract excerpt

OBJECTIVE: To develop a multiplex polymerase chain reaction (PCR) assay to detect the genes for the major toxins of Clostridium perfringens (cpa [alpha toxin], cpb [beta toxin], etx [epsilon toxin], iA [iota toxin], and cpe [enterotoxin]). SAMPLE POPULATION: Cultures of C perfringens obtained from collections and diagnosticians throughout North America. PROCEDURE: PCR primers were derived from published sequences...

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PMID: 9215442

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