Article
Species variation within the internal transcribed spacer (ITS) region of Gyrodactylus (Monogenea:Gyrodactylidae) ribosomal RNA genes.
The Journal of parasitology - 1 Apr 1997
Cunningham C O
Abstract excerpt
The ribosomal internal transcribed spacer (ITS) region from individual Gyrodactylus specimens was amplified by polymerase chain reaction (PCR). The reaction amplified the entire ITS1-5.8S-ITS2 region of the ribosomal RNA gene cluster using primers that hybridize to the 3' terminus of the small su...
Topics
- Animals
- Base Sequence
- Cestoda
- Cestode Infections
- Cloning, Molecular
- Fish Diseases
- Fishes
- Genetic Variation
- Molecular Sequence Data
- Multigene Family
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- RNA, Helminth
- RNA, Ribosomal
- Schistosoma
- Sequence Alignment
- Sequence Homology, Nucleic Acid