Article
Random insertion of transposon Tn4001 in the genome of Spiroplasma citri strain GII3.
Plasmid - 1 Jan 1997
Foissac X, Saillard C, Bové J M
Abstract excerpt
Electroporation of Spiroplasma citri strain GII3 with plasmid pMUT containing the Staphylococcus aureus transposon Tn4001 resulted in random insertion of Tn4001 into the spiroplasmal genome. Transformation frequencies reached 10(-8) per colony-forming unit (CFU) when 100 microg of plasmid DNA and 3 x 10(9) S. citri CFU were used. Three other strains of S. citri failed to be transformed under the same conditions....
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