Random insertion of transposon Tn4001 in the genome of Spiroplasma citri strain GII3.

doi:10.1006/plas.1996.1271

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Article

Random insertion of transposon Tn4001 in the genome of Spiroplasma citri strain GII3.

Plasmid - 1 Jan 1997

Foissac X, Saillard C, Bové J M

Abstract excerpt

Electroporation of Spiroplasma citri strain GII3 with plasmid pMUT containing the Staphylococcus aureus transposon Tn4001 resulted in random insertion of Tn4001 into the spiroplasmal genome. Transformation frequencies reached 10(-8) per colony-forming unit (CFU) when 100 microg of plasmid DNA and...

Topics

Anti-Bacterial Agents
DNA Transposable Elements
Drug Resistance, Microbial
Electroporation
Escherichia coli
Genome, Bacterial
Gentamicins
Mutation
Spiroplasma
Staphylococcus aureus
Transformation, Genetic

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