Article
Recombinant type A and B phytochromes from potato. Transient absorption spectroscopy.
Biochemistry - 7 Jan 1997
Ruddat A, Schmidt P, Gatz C, Braslavsky S E, Gärtner W, Schaffner K
Abstract excerpt
The cDNAs encoding full-length type A and B phytochromes (phyA and phyB, respectively) from potato were expressed in inducible yeast systems (Saccharomyces cerevisiae and Pichia pastoris). In addition, a deletion mutant of phyB (delta 1-74) was expressed. The apoproteins were reconstituted into chromoproteins by incorporation of the native chromophore, phytochromobilin (P phi B), and of phycocyanobilin (PCB). The...
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