Recombinant type A and B phytochromes from potato. Transient absorption spectroscopy.

doi:10.1021/bi962012w

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Article

Recombinant type A and B phytochromes from potato. Transient absorption spectroscopy.

Biochemistry - 7 Jan 1997

Ruddat A, Schmidt P, Gatz C, Braslavsky S E, Gärtner W, Schaffner K

Abstract excerpt

The cDNAs encoding full-length type A and B phytochromes (phyA and phyB, respectively) from potato were expressed in inducible yeast systems (Saccharomyces cerevisiae and Pichia pastoris). In addition, a deletion mutant of phyB (delta 1-74) was expressed. The apoproteins were reconstituted into c...

Topics

Biliverdine
Blotting, Western
Cloning, Molecular
Gene Expression
Kinetics
Molecular Structure
Mutation
Photolysis
Phycobilins
Phycocyanin
Phytochrome
Pichia
Plant Proteins
Polymerase Chain Reaction
Pyrroles
Recombinant Proteins
Saccharomyces cerevisiae
Sequence Deletion

Source

Open in PubMed

Identifiers

PMID: 8993323
DOI: 10.1021/bi962012w

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8993323-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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