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Article

Recovery of cytopathogenic and noncytopathogenic bovine viral diarrhea viruses from cDNA constructs

1 Dec 1996

Abstract excerpt

After cDNA cloning of the genome of bovine viral diarrhea virus (BVDV) isolate CP7, a full-length cDNA clone was constructed. RNA transcribed in vitro from this construct was shown to direct the generation of infectious BVDV upon transfection into bovine cells. To confirm the de novo generation of infectious BVDV from cloned cDNA a genetically tagged virus was constructed. In comparison with parental BVDV, the...

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PMID: 8970985
DOI: 10.1128/jvi.70.12.8606-8613.1996

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