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In Vitro Developmental Competence of in Vitro-Matured Bovine Oocytes Fertilized and Cultured in Completely Defined Media1

1 Aug 1996

Abstract excerpt

The objective was to establish an in vitro system in which bovine oocytes can be matured, fertilized, and cultured up to the blastocyst stage without support of serum, BSA, or somatic cells. Media consisted of modified tissue culture medium 199 (mTCM 199) with ovine LH (oLH) for maturation (IVM), experimental alterations of modified defined medium (mDM) for sperm selection and insemination (IVF), and...

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PMID: 8828837
DOI: 10.1095/biolreprod55.2.333

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