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Detection and analysis of diverse herpesviral species by consensus primer PCR

1 Jul 1996

Abstract excerpt

A consensus primer PCR method which amplifies a region of herpesviral DNA-directed DNA polymerase (EC 2.7.7.7) and which uses degenerate primers in a nested format was developed. Primers were designed to target sequences coding for highly conserved amino acid motifs covering a region of approximately 800 bp. The assay was applied to 22 species of herpesviruses (8 human and 14 animal viruses), with PCR products...

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PMID: 8784566
DOI: 10.1128/jcm.34.7.1666-1671.1996

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