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Article

Cloning and characterization of the metE gene encoding S-adenosylmethionine synthetase from Bacillus subtilis.

Journal of bacteriology - 1 Aug 1996

Yocum R R, Perkins J B, Howitt C L, Pero J

Abstract excerpt

The metE gene, encoding S-adenosylmethionine synthetase (EC 2.5.1.6) from Bacillus subtilis, was cloned in two steps by normal and inverse PCR. The DNA sequence of the metE gene contains an open reading frame which encodes a 400-amino-acid sequence that is homologous to other known S-adenosylmethionine synthetases. The cloned gene complements the metE1 mutation and integrates at or near the chromosomal site of...

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Identifiers

PMID: 8755891
DOI: 10.1128/jb.178.15.4604-4610.1996
PMCID: PMC178230

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