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Article

Concordance of clinical and environmental isolates of Cryptococcus neoformans var. gattii by random amplification of polymorphic DNA analysis and PCR fingerprinting.

Journal of clinical microbiology - 1 May 1996

Sorrell T C, Chen S C, Ruma P, Meyer W, Pfeiffer T J, Ellis D H, Brownlee A G

Abstract excerpt

Sixty-one clinical and forty-nine environmental isolates of Cryptococcus neoformans var. gattii from Australia and the United States were analyzed by random amplification of polymorphic DNA (RAPD), using 12- to 22-mer primers in pairs, and/or PCR fingerprinting with a single primer derived from the microsatellite core sequence of the wild-type phage M13 (5' GAGGGTGGCGGTTCT 3'). Three major genetic profiles were...

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Identifiers

PMID: 8727912
DOI: 10.1128/jcm.34.5.1253-1260.1996
PMCID: PMC228991

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