Article
Purification and sequencing of multiple forms of Brassica napus seed napin small chains that are calmodulin antagonists and substrates for plant calcium-dependent protein kinase.
Biochimica et biophysica acta - 7 Jun 1996
Neumann G M, Condron R, Thomas I, Polya G M
Abstract excerpt
Six napin small (S) subunits and six napin large (L) subunits were resolved from the seeds of kohlrabi (Brassica napus var. rapifera) by a procedure involving extraction, batchwise elution from carboxymethylcellulose (CM52) and reverse-phase HPLC after treatment with guanidine hydrochloride and 2...
Topics
- 2S Albumins, Plant
- Amino Acid Sequence
- Brassica
- Calmodulin
- Genetic Variation
- Molecular Sequence Data
- Molecular Weight
- Myosin-Light-Chain Kinase
- Peptide Fragments
- Phosphorylation
- Plant Proteins
- Protein Kinases
- Protein Structure, Secondary
- Seeds
- Sequence Analysis