Article
Expression, purification, and characterization of EpiC, an enzyme involved in the biosynthesis of the lantibiotic epidermin, and sequence analysis of Staphylococcus epidermidis epiC mutants.
Journal of bacteriology - 1 Mar 1996
Kupke T, Gotz F
Abstract excerpt
The plasmid-encoded epidermin biosynthetic gene epiC of Staphylococcus epidermidis Tü3298 was expressed in Escherichia coli by using the T7 RNA polymerase-promoter system, and the gene product EpiC was identified by Western blotting (immunoblotting) with an anti-EpiC-peptide antiserum. EpiC was a...
Topics
- Amino Acid Sequence
- Anti-Bacterial Agents
- Bacterial Proteins
- Bacteriocins
- Base Sequence
- Chromatography, High Pressure Liquid
- Cloning, Molecular
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Peptides
- Protein Binding
- Protein Precursors
- Recombinant Proteins
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- Staphylococcus epidermidis