Article
Hordein promoter methylation and transcriptional activity in wild-type and mutant barley endosperm.
Molecular & general genetics : MGG - 10 Apr 1996
Sørensen M B, Müller M, Skerritt J, Simpson D
Abstract excerpt
B- and C-hordein gene transcription is severely reduced in the endosperm of the regulatory barley mutant lys3a, and this is correlated with persistent hypermethylation of the promoters. In contrast, D-hordein is expressed at normal levels in the mutant. To confirm the connection between methylati...
Topics
- Base Sequence
- Blotting, Southern
- Blotting, Western
- Cloning, Molecular
- DNA Primers
- Gene Expression Regulation, Plant
- Genes, Reporter
- Glucuronidase
- Glutens
- Hordeum
- Immunohistochemistry
- Methylation
- Molecular Sequence Data
- Mutation
- Plant Proteins
- Polymorphism, Genetic
- Promoter Regions, Genetic
- Transcription, Genetic