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Article

High-GC primers are useful in RAPD analysis of fungi.

Current genetics - 1 Sept 1995

Kubelik A R, Szabo L J

Abstract excerpt

For genetic analysis of fungal DNAs, we have modified the RAPD method to use primers with G + C contents of 80-100%. In RAPD analysis of Puccinia graminis f. sp. tritici DNAs, these primers generated twice the number of both amplification products per primer and polymorphisms among isolates as compared to the standard 60-70% G + C primers. With respect to segregation and genetic similarity, RAPD markers generated...

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PMID: 8590486
DOI: 10.1007/BF00326438

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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