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Rapid Determination on Sperm Cell Concentration in Bovine Semen by Flow Cytometry

1 Jan 1993

Abstract excerpt

A flow cytometric technique is described for determining sperm concentration in fresh or extended semen with improved accuracy, precision, repeatability, ease of conduct, and rapidity. The technique is designed to measure the ratio of a known number of fluorescent beads admixed with sperm stained with either acridine orange or propidium iodide. A significant advantage of the technique is the distinct resolution...

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PMID: 8436684
DOI: 10.3168/jds.s0022-0302(93)77326-9

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