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Identification of Mycobacterium species by using amplified ribosomal DNA restriction analysis

1 Aug 1993

Abstract excerpt

A rapid procedure for the identification of cultured Mycobacterium isolates, based on the combination of enzymatic amplification and restriction analysis, is described. The 16S rRNA genes (rDNA) of 99 strains belonging to 18 different species of the genus Mycobacterium were enzymatically amplified. Amplified rDNA restriction analysis with the enzymes CfoI, MboI, and RsaI was carried out. The combination of the...

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PMID: 8396586
DOI: 10.1128/jcm.31.8.2061-2065.1993

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