Article
Generation of restriction map of Enterococcus faecalis OG1 and investigation of growth requirements and regions encoding biosynthetic function.
Journal of bacteriology - 1 Aug 1993
Murray B E, Singh K V, Ross R P, Heath J D, Dunny G M, Weinstock G M
Abstract excerpt
A defined synthetic medium was used to determine the amino acid requirements of Enterococcus faecalis OG1 and to demonstrate the absence of a requirement for exogenous purines or pyrimidines. Genomic libraries prepared from strain OG1 were transduced into Escherichia coli auxotrophic mutants, and...
Topics
- Amino Acids
- Cloning, Molecular
- Culture Media
- Deoxyribonucleases, Type II Site-Specific
- Enterococcus faecalis
- Escherichia coli
- Gene Library
- Genetic Complementation Test
- Genome, Bacterial
- Mutation
- Restriction Mapping