Article
Detection of electrophoretic variants of Notch, PS integrin, and DROP-1 proteins in Drosophila following extraction in guanidine hydrochloride.
Biochemical and biophysical research communications - 15 Jul 1993
Zackson S L, Graner M W, Karr T L
Abstract excerpt
A method is presented for the rapid extraction of proteins from Drosophila tissues. This method involves lysis of embryos in high concentrations of guanidine hydrochloride, followed by ultracentrifugation in a guanidine hydrochloride step gradient. Several membrane-associated antigens, including Notch and the beta subunit of PS integrin are enriched in this preparation. The quantity of the proteoglycan, DROP-1,...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
