[The variability of the pattern of human G-band chromosomes in the late prophase-early metaphase].

Abstract excerpt

Original modification of the harvest procedure, hypotonic treatment and slide-making techniques were used to obtain prometaphase spreads of good quality fitted to G-banding or to FISH. Human blood cultures were synchronized with a methotrexate block during synthesis, and following this with a thymidine release. Cells were then proceeded into prometaphase and early metaphase and were quickly harvested without...

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