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Polymerase chain reaction detection of Plasmodium falciparum in mosquitoes

1 May 1993

Abstract excerpt

The polymerase chain reaction (PCR) procedure using a primer set derived from a repetitive deoxyribonucleic acid (DNA) sequence specific to Plasmodium falciparum was used to detect parasite DNA in mosquitoes. In laboratory-infected mosquitoes, PCR could detect as few as 10 sporozoites in a dissected salivary gland and a single oocyst in a dissected midgut. The ability to detect P. falciparum DNA in wild-caught...

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PMID: 8236389
DOI: 10.1016/0035-9203(93)90124-9

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