Article
Giardia intestinalis: detection of major genotypes by restriction analysis of gene amplification products.
International journal for parasitology - 1 Aug 1993
Ey P L, Darby J M, Andrews R H, Mayrhofer G
Abstract excerpt
The polymerase chain reaction (PCR) has been used to amplify a 0.52 kb segment of Giardia intestinalis DNA, using primers specific for nucleotide sequences conserved within two genes (tsp11 and tsa417) that encode homologous, cysteine-rich trophozoite surface proteins. Using products amplified from axenic isolates belonging to genetic groups I and II (defined on the basis of allozyme electrophoresis data),...
Topics
- Animals
- Antigens, Protozoan
- Base Sequence
- Cloning, Molecular
- DNA Probes
- Genes, Protozoan
- Genotype
- Giardia lamblia
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Polymerase Chain Reaction