Article
Giardia intestinalis: detection of major genotypes by restriction analysis of gene amplification products.
International journal for parasitology - 1 Aug 1993
Ey P L, Darby J M, Andrews R H, Mayrhofer G
Abstract excerpt
The polymerase chain reaction (PCR) has been used to amplify a 0.52 kb segment of Giardia intestinalis DNA, using primers specific for nucleotide sequences conserved within two genes (tsp11 and tsa417) that encode homologous, cysteine-rich trophozoite surface proteins. Using products amplified from axenic isolates belonging to genetic groups I and II (defined on the basis of allozyme electrophoresis data),...
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