Enhanced Rates of Cleavage and Development for Sheep Zygotes Cultured to the Blastocyst Stage in Vitro in the Absence of Serum and Somatic Cells: Amino Acids, Vitamins, and Culturing Embryos in Groups Stimulate Development1

Abstract excerpt

The aim of this study was to develop a serum-free culture system that could support high levels of cleavage and blastocyst formation from sheep zygotes developed in vitro. To this end, we investigated the effects on sheep zygote development of amino acids, ammonium, vitamins, and culture of embryos in groups in Synthetic Oviduct Fluid (SOF) medium supplemented with BSA (32 mg/ml). The inclusion of amino acids in...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Animal Genetics and Reproduction
• Share to Pluripotent Stem Cells Research