Article
Species differentiation in the Didymozoidae (Digenea): restriction fragment length differences in internal transcribed spacer and 5.8S ribosomal DNA.
International journal for parasitology - 1 Feb 1993
Anderson G R, Barker S C
Abstract excerpt
Genetic differences among six didymozoid species distinguished on morphological criteria (Didymozoon species A, B, E, F and G, and Neometadidymozoon helicis) were sought by restriction fragment length analysis of internal transcribed spacer (ITS) and 5.8S ribosomal DNA. DNA fragments comprising ITS1, 5.8S and ITS2 were amplified by polymerase chain reaction for each of the six species. Digestion with the...
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