Identification of S-locus linked PCR fragments in rye (Secale cereale L.) by denaturing gradient gel electrophoresis.

doi:10.1046/j.1365-313x.1994.5060891.x

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Article

Identification of S-locus linked PCR fragments in rye (Secale cereale L.) by denaturing gradient gel electrophoresis.

The Plant journal : for cell and molecular biology - 1 Jun 1994

Wehling P, Hackauf B, Wricke G

Abstract excerpt

Rye inbred lines segregating at the S-locus and homozygous at the Z-locus were investigated by PCR with primers derived from Brassica SLG-sequences. After denaturing gradient gel electrophoresis (DGGE), a 280 bp PCR-fragment displays a polymorphism perfectly correlated to the underlying S-genotyp...

Topics

Alleles
Amino Acid Sequence
Base Sequence
Brassica
Electrophoresis, Polyacrylamide Gel
Genetic Techniques
Genotype
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Genetic
Secale

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