Article
A new gene expression system based on a fructose-dependent promoter from Rhodobacter capsulatus.
Gene - 22 Jul 1994
Duport C, Meyer C, Naud I, Jouanneau Y
Abstract excerpt
Translational lacZ fusions were constructed to analyse transcription of the fructose operon, encoding the fructose-specific phosphotransferase system of Rhodobacter capsulatus. It was demonstrated that transcription from the fru promoter (fruP) was negligible without fructose, and stimulated more than 100-fold by the presence of the inducer. A multiple cloning site, fruP, and a cassette conferring gentamycin...
Topics
- Base Sequence
- Binding Sites
- Cloning, Molecular
- DNA, Bacterial
- Fructose
- Genetic Complementation Test
- Genetic Vectors
- Molecular Sequence Data
- Mutation
- Operon
- Promoter Regions, Genetic