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Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis.

Archives of virology - 1 Jan 1994

Vilcek S, Herring A J, Herring J A, Nettleton P F, Lowings J P, Paton D J

Abstract excerpt

A polymerase chain reaction-based assay capable of detecting a broad range of pestiviruses from pigs, cattle, or sheep was developed. Of six sets of primers selected from different parts of the pestivirus genome, the best results were provided by a pair from the highly conserved 5' non-coding region which gave amplification with all 129 isolates tested. This panel consisted of 33 isolates from pigs, 79 from...

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PMID: 8031236
DOI: 10.1007/BF01321060

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