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Article

Improved sizing of fragile X CCG repeats by nested polymerase chain reaction.

American journal of medical genetics - 15 Jul 1994

Levinson G, Maddalena A, Palmer F T, Harton G L, Bick D P, Howard-Peebles P N, Black S H, Schulman J D

Abstract excerpt

We have developed an improved method for polymerase chain reaction (PCR)-based sizing of the CCG repeat region at the fragile X locus, FMR-1. This method is designed to optimize denaturation and replication of long repeats with high G + C content, which are otherwise refractory to amplification. The method utilizes nested PCR primers to increase sensitivity and specificity. Alkaline denaturation of the genomic...

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PMID: 7943035
DOI: 10.1002/ajmg.1320510448

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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