Article
Improved sizing of fragile X CCG repeats by nested polymerase chain reaction.
American journal of medical genetics - 15 Jul 1994
Levinson G, Maddalena A, Palmer F T, Harton G L, Bick D P, Howard-Peebles P N, Black S H, Schulman J D
Abstract excerpt
We have developed an improved method for polymerase chain reaction (PCR)-based sizing of the CCG repeat region at the fragile X locus, FMR-1. This method is designed to optimize denaturation and replication of long repeats with high G + C content, which are otherwise refractory to amplification....
Topics
- Base Sequence
- DNA Mutational Analysis
- DNA Primers
- Electrophoresis, Polyacrylamide Gel
- Female
- Fragile X Syndrome
- Gene Dosage
- Genetic Testing
- Humans
- Male
- Molecular Sequence Data
- Mutation
- Nucleic Acid Denaturation
- Polymerase Chain Reaction
- Repetitive Sequences, Nucleic Acid