Article
Cloning, characterization, and genetics of the juvenile hormone esterase gene from Heliothis virescens.
Insect biochemistry and molecular biology - 1 Jul 1994
Harshman L G, Ward V K, Beetham J K, Grant D F, Grahan L J, Zraket C A, Heckel D G, Hammock B D
Abstract excerpt
The gene for juvenile hormone esterase (JHE) was cloned from Heliothis virescens (Lepidoptera: Noctuidae). A genomic library was constructed from embryonic DNA and screened with a homologous N-terminal probe from the JHE cDNA. Five genomic clones were isolated and analyzed by dot blot hybridization using regions of the JHE cDNA as probes. Clone C hybridized to both 5' and 3' probes from the JHE cDNA, suggesting...
Topics
- Animals
- Base Sequence
- Carboxylic Ester Hydrolases
- Cloning, Molecular
- Crosses, Genetic
- DNA Primers
- DNA Probes
- Deoxyribonucleases, Type II Site-Specific
- Embryo, Nonmammalian
- Female
