Article
Non-motile mutants of Helicobacter pylori and Helicobacter mustelae defective in flagellar hook production.
Molecular microbiology - 1 Nov 1994
O'Toole P W, Kostrzynska M, Trust T J
Abstract excerpt
Flagellar hooks were purified from Helicobacter pylori and Helicobacter mustelae. The 70 x 16 nm H. pylori hook was composed of FlgE subunits of 78kDa, while the 72 x 16 nm H. mustelae hook was composed of 87 kDa subunits. N-terminal sequence was obtained for the FlgE proteins of both species, and for an internal H. mustelae FlgE peptide. Degenerate oligonucleotide primers allowed amplification of a 1.2 kb...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Cell Movement
- Cloning, Molecular
- DNA Primers
- DNA, Bacterial
- Flagella
- Flagellin
- Gene Amplification
- Genes, Bacterial