Article
Oligonucleotide fingerprinting detects genetic diversity among Ascochyta rabiei isolates from a single chickpea field in Tunisia.
Current genetics - 1 Sept 1994
Morjane H, Geistlinger J, Harrabi M, Weising K, Kahl G
Abstract excerpt
Fifty isolates of Ascochyta rabiei (Pass.) Labr. were hierarchically sampled from four well-separated locations of a single chickpea field in Beja (Tunisia), and single-spored. DNA was isolated from in-vitro-grown mycelia, digested with HinfI or RsaI, and hybridized to a set of synthetic oligonucleotides complementary to simple repetitive sequences. According to the fingerprint patterns derived from the probes...
Topics
- Fabaceae
- Genetic Heterogeneity
- Genetic Variation
- Genotype
- Haplotypes
- Mitosporic Fungi
- Nucleotide Mapping
- Oligonucleotide Probes
- Plants, Medicinal
- Polymorphism, Genetic
- Reproducibility of Results
