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Article

Development of a bovine adenovirus type 3-based expression vector

1 Jan 1995

Abstract excerpt

We constructed a non-defective bovine adenovirus type 3 recombinant (BAd3-Luc) containing the firefly luciferase gene inserted in the early region 3 (E3) of the BAd3 genome. Deletion of a 696 bp XhoI-NcoI E3 segment and insertion of the luciferase gene in E3 was confirmed by Southern blot analyses. Luciferase was expressed in Madin-Darby bovine kidney cells infected with BAd3-Luc as measured by enzymic assays and...

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Identifiers

PMID: 7844546
DOI: 10.1099/0022-1317-76-1-93

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