Article
Extensive mtDNA diversity in horses revealed by PCR-SSCP analysis.
Animal genetics - 1 Jun 1995
Marklund S, Chaudhary R, Marklund L, Sandberg K, Andersson L
Abstract excerpt
The hypervariable D-loop region of mitochondrial DNA (mtDNA) was amplified with the polymerase chain reaction using total horse DNA samples. Analysis of single strand conformation polymorphism (SSCP) of denatured amplification products was carried out by native polyacrylamide (8%) gel electrophoresis followed by silver staining. As many as 15 distinct SSCP variants were revealed when screening a total of 78...
Topics
- Animals
- Base Sequence
- DNA Primers
- DNA, Mitochondrial
- Female
- Genetic Variation
- Genomic Imprinting
- Horses
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Single-Stranded Conformational
