[The effect of the duration of culturing on the pluripotency of mouse embryonic stem (ES) cells in vitro and in vivo].

Article

[The effect of the duration of culturing on the pluripotency of mouse embryonic stem (ES) cells in vitro and in vivo].

Ontogenez - 1 Jan 2000

Mitalipov Sh M, Mitallipova M M, Ivanov V I

Abstract excerpt

A comparative analysis of two clones of mouse embryonic stem cells (ES-D3) that underwent different number of passages was performed to determine their potencies for in vitro and in vivo development. Cells of both clones had similar morphology characteristic of undifferentiated ES cells and were...

Topics

Alkaline Phosphatase
Animals
Blastocyst
Cell Line
Chimera
Clone Cells
Embryo Transfer
Female
Genotype
Karyotyping
Male
Mice
Mice, Inbred Strains
Stem Cells
Time Factors

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