Article
[Genetic analysis of the genotype of ABO blood group with the DNA from a hair].
Rinsho byori. The Japanese journal of clinical pathology - 1 Apr 1995
Hosoi E
Abstract excerpt
The genotype of the hair genomic DNA from 14 subjects with the ABO blood group was determined using the polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method. The amino acid substitutions of codon 87 and 176 of ABO allelic cDNAs were analyzed to distinguish A, B, and O alleles by restriction enzyme digestion. To identify codon 87, the 249bp DNA fragment was amplified by PCR and...
Topics
- ABO Blood-Group System
- Adult
- Alleles
- Base Sequence
- Codon
- DNA, Complementary
- Genotype
- Hair
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
