Article
Effect of cellular physiology on PCR amplification efficiency.
Molecular ecology - 1 Feb 1995
Silva M C, Batt C A
Abstract excerpt
Culture conditions, and other variables that modulate a cell's physiology, can bias a polymerase chain reaction (PCR) amplification against generating a representative population profile. Two Pseudomonas putida nahR alleles were constructed in pUC19 that differ solely in a 31-bp internal segment...
Topics
- Alleles
- Base Sequence
- Cloning, Molecular
- Culture Media
- DNA Primers
- DNA, Bacterial
- Ecosystem
- Escherichia coli
- Gene Amplification
- Genes, Bacterial
- Molecular Sequence Data
- Mutagenesis
- Naphthalenes