Article
Effect of cellular physiology on PCR amplification efficiency.
Molecular ecology - 1 Feb 1995
Silva M C, Batt C A
Abstract excerpt
Culture conditions, and other variables that modulate a cell's physiology, can bias a polymerase chain reaction (PCR) amplification against generating a representative population profile. Two Pseudomonas putida nahR alleles were constructed in pUC19 that differ solely in a 31-bp internal segment whose sequence has been inverted. After PCR amplification, the products could be distinguished on the basis of a change...
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