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Species Identification of Avian Mycoplasmas by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism Analysis

1 Apr 1995

Abstract excerpt

Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis were used to detect and differentiate four pathogenic species (Mycoplasma gallisepticum, M. iowae, M. meleagridis, and M. synoviae) and ten nonpathogenic species of avian mycoplasma. A sequence of 1026 base pairs within the gene for 16S ribosomal RNA (16S rRNA) from avian mycoplasmas was successfully amplified by PCR with...

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PMID: 7677664
DOI: 10.2307/1591885

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