Article
Tryptophan-scanning mutagenesis of MotB, an integral membrane protein essential for flagellar rotation in Escherichia coli.
Biochemistry - 18 Jul 1995
Sharp L L, Zhou J, Blair D F
Abstract excerpt
The MotB protein of Escherichia coli is an essential component of the flagella that functions together with the MotA protein in transmembrane proton conduction. MotB has a single hydrophobic segment that spans the membrane. In order to determine which parts of the membrane-spanning segment can tolerate the introduction of a large, hydrophobic side chain, single Trp residues were substituted into many consecutive...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Escherichia coli
- Flagella
- Membrane Proteins
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Protein Conformation
- Protons