Article
Genetic diversity within mer genes directly amplified from communities of noncultivated soil and sediment bacteria.
Molecular ecology - 1 Oct 1995
Bruce K D, Osborn A M, Pearson A J, Strike P, Ritchie D A
Abstract excerpt
Individual merRT delta P regions were amplified from DNA directly isolated from soil and sediment samples using consensus primers derived from the conserved mer sequences of Tn501, Tn21 and pMER419. Soil and sediment samples were taken from four sites in the British Isles; one 'pristine' (SB) and three polluted (SO, SE, T2) with respect to mercury. The sizes of the PCR products amplified (approximately 1 kb) were...
Topics
- Bacteria
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- Consensus Sequence
- Conserved Sequence
- DNA Primers
- DNA Transposable Elements
- DNA, Bacterial
- Genes, Bacterial
- Genetic Variation
