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Article

Genetic diversity within mer genes directly amplified from communities of noncultivated soil and sediment bacteria.

Molecular ecology - 1 Oct 1995

Bruce K D, Osborn A M, Pearson A J, Strike P, Ritchie D A

Abstract excerpt

Individual merRT delta P regions were amplified from DNA directly isolated from soil and sediment samples using consensus primers derived from the conserved mer sequences of Tn501, Tn21 and pMER419. Soil and sediment samples were taken from four sites in the British Isles; one 'pristine' (SB) and three polluted (SO, SE, T2) with respect to mercury. The sizes of the PCR products amplified (approximately 1 kb) were...

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PMID: 7582168
DOI: 10.1111/j.1365-294x.1995.tb00260.x

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