Use of restriction fragment polymorphism analysis of rRNA genes to assign species to unknown clinical isolates of oral viridans streptococci.

Abstract excerpt

This study evaluated restriction fragment length polymorphisms of rRNA genes (ribotyping) for genotypic identification of 53 oral isolates classified as "Streptococcus sanguis" by colony morphology. Isolates were from 8-h buccal plaque on lower first permanent molars of 20 subjects. DNA was digested with AatII and hybridized with digoxygenin-labeled cDNA of Escherichia coli 16S and 23S rRNA. Strains were...

Topics

• Bacterial Typing Techniques
• Dental Plaque
• Genes, Bacterial
• Humans
• Phenotype
• Polymorphism, Restriction Fragment Length
• RNA, Bacterial
• RNA, Ribosomal
• Reference Standards
• Species Specificity
• Streptococcus