Article
[Location of the reconstitution initiation sites of TMV Ni2519 RNA].
Molekuliarnaia biologiia - 1 Jan 2000
Kaplan I B, Pshennikova E S, Kozlov Iu V, Agranovskiĭ A A, Tal'ianskiĭ M E
Abstract excerpt
The nature of temperature sensitivity (ts) of TMV mutant Ni2529 has been studied in experiments on in vitro reconstitution. At permissive temperature (24 degrees C) RNA of Ni2519 can be reassembled into an infective virus. At nonpermissive temperature (33 degrees C) the reassembly of Ni2519 results in defective (ribonuclease-sensitive) particle formation. The RNase-sensitive site of Ni2519 RNA molecule in the...
Topics
- Microscopy, Electron
- Mutation
- RNA, Viral
- Ribonucleases
- Temperature
- Tobacco Mosaic Virus