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Development and application of a dual RT-qPCR assay for the differential detection of bovine rhinitis virus genotypes A and B.

Journal of virological methods - 1 Sept 2026

Liu Libing, Xiang Jialin, Shi Yunpeng, Cao Binglei, Gao Yixiang, Wang Jinfeng, Sun Xiaoxia, Wang Jianchang

Abstract excerpt

Bovine rhinitis virus (BRV) is an important pathogen causing bovine respiratory disease complex (BRDC). To facilitate the rapid and simultaneous detection of bovine rhinitis A virus (BRAV) and bovine rhinitis B virus (BRBV), specific primers and TaqMan probes targeting the viral 3D gene were designed and synthesized. Following the optimization of primer and probe concentrations and annealing temperatures, as well...

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PMID: 42248256
DOI: 10.1016/j.jviromet.2026.115425

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