Article
Comparison of 18S rRNA gene fragments and reference databases for assessing bovine Eimeria diversity using the Illumina platform.
Veterinary parasitology - 1 Jul 2026
Lee Subin, Youn So Youn, Seo Min-Goo, Yoo Mi-Sun, Lee Hyang-Sim, Cho Yun Sang, Kwak Dongmi, Lee Seung-Hun
Abstract excerpt
This study compared the effects of different 18S rRNA target regions and reference databases on assessing the diversity of bovine Eimeria spp. using metabarcoding. A total of 32 diarrheic bovine fecal samples, confirmed positive for Eimeria through microscopy and PCR, were collected in Korea. The DNA from each sample was pooled for DNA metabarcoding, which targeted the V4 and V9 regions of the 18S rRNA gene, as...
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