Article
Development of the first specific real-time PCR TaqMan assay for fowl adenovirus serotype 11 diagnosis.
Avian pathology : journal of the W.V.P.A - 1 Aug 2026
Kardoudi Amina, Siham Fellahi, Abdelmounaaim Allaoui, Faouzi Kichou, Ikram Ouchhour, Drissi Touzani Charifa, Thomas Jackson, Abdelouaheb Benani
Abstract excerpt
RESEARCH HIGHLIGHTS: Development of the first specific real-time PCR TaqMan assay for FAdV-11 detection, quantification, and genotyping in one reaction.Excellent linearity (R2 = 0.995) and analytical sensitivity of 10 copies/µl.Specific amplification of FAdV-11 with no cross-reactivity with other FAdV serotypes or avian pathogens.Clinical validation on IBH field samples showed perfect concordance with sequencing...
Topics
- Animals
- Real-Time Polymerase Chain Reaction
- Poultry Diseases
- Serogroup
- Chickens
- Sensitivity and Specificity
- Adenoviridae Infections
- Aviadenovirus
- Genotype
