Molecular and phenotypic characterization of carbapenemase-producing Klebsiella pneumoniae and Acinetobacter baumannii from bovine mastitis milk with development of a novel optimized spectrophotometric UniCarb assay.

Abstract excerpt

BACKGROUND: Carbapenemase-producing Gram-negative bacteria are increasingly reported in veterinary medicine, posing a serious threat to both animal and public health. This study investigated phenotypic and molecular mechanisms of carbapenem resistance in Klebsiella pneumoniae and Acinetobacter baumannii isolated from bovine mastitis milk. METHODS: Antimicrobial susceptibility testing, minimum inhibitory...

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