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Article

A pennycress transparent testa 8 knockout mutant has drastic changes in seed coat anatomy and chemical compositions.

Journal of experimental botany - 27 Mar 2026

Ding Xinxin, Duckworth Summer, Southworth Madeline, Lipton Andrew, Clendinen Chaevien S, Gautam Barsanti, Esfahanian Maliheh, Velickovic Dusan, Sedbrook John C, Handakumbura Pubudu

Abstract excerpt

Pennycress is a winter annual intermediate crop with ∼30% seed oil content suitable for producing biofuels. Here, we evaluated seed development, anatomy, and agronomically relevant traits of a transparent testa 8 knockout mutant (tt8-2bp) generated by CRISPR genome editing to improve seed quality. We performed histochemical analyses on wild-type and tt8-2bp seeds at different developmental stages. No visible...

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Identifiers

PMID: 41685867
DOI: 10.1093/jxb/erag078
PMCID: PMC13022552

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