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A direct comparison of STS-PCR and RFLP for detection and subtyping of human Blastocystis in Iran.

Diagnostic microbiology and infectious disease - 1 May 2026

Karimi Ehsan, Momeni Zohreh, Nasiri Vahid, Sabokbar Azar

Abstract excerpt

Blastocystis is a genetically diverse unicellular eukaryotic intestinal parasite with no gold-standard diagnostic method. This study, conducted in Iran, is the first to directly compare Sequence-Tagged Site (STS) PCR and restriction fragment length polymorphism (RFLP) for its diagnosis of human. It builds on our previous work with the same cohort. We aimed to evaluate the diagnostic sensitivity and specificity of...

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PMID: 41643591
DOI: 10.1016/j.diagmicrobio.2026.117265

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STS

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