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Developmental validation of the SF 28CS typing system: a robust 6-dye multiplex for forensic human identification.

International journal of legal medicine - 1 Mar 2026

Adnan Atif, Lei Zhang, Rakha Allah, Jiajia Guo, Zhuoying Zhu, Yixin Wu, Jiawen Yang, Hameed Muhammad, Nazir Shahid, Shen Qu, Wang Chuan-Chao, Wang Hongbo

Abstract excerpt

Short tandem repeats (STRs) are central to human identification and kinship analysis owing to their high polymorphism. We report the developmental validation of the SF28CS typing system, a novel six-dye multiplex PCR assay that simultaneously amplifies 28 loci, including 23 autosomal STRs (18 expanded CODIS loci, Penta D, Penta E, and three additional markers), three Y-STRs (DYS391, DYS576, DYS518), one...

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PMID: 41389099
DOI: 10.1007/s00414-025-03653-5

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