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Mycoplasma agassizii Multilocus Sequence Typing Using Nanopore Sequencing: Insights Into Genetic Diversity and Isolate Characterization.

Annals of the New York Academy of Sciences - 1 Dec 2025

Cacciotto Carla, Zobba Rosanna, Louro Manuel, Alves Margarida, Valença Andreia, Patrício Rui, Bazzoni Emanuela, Pittau Marco, Lecis Roberta, Alberti Alberto

Abstract excerpt

Mycoplasma agassizii (Mycoplasmopsis agassizii) is a major pathogen responsible for upper respiratory tract disease (URTD) in tortoises, contributing to worldwide population declines. Despite its significance, tools for strain-level identification and epidemiological tracking remain limited. This study proposes a Multilocus Sequence Typing (MLST) scheme based on eight housekeeping genes (uvrB, tpiA, gyrB, efTu,...

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PMID: 41168653
DOI: 10.1111/nyas.70128
PMCID: PMC12728326

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